Distribution ratios (i.e., the ratio of intracellular concentration of radiolabel relative to the extracellular concentration) were calculated as described previously. falciparum trophozoites at increasing concentrations of WU-1. Data are expressed as means ± SEMs of three independent experiments performed in duplicate. falciparum strain 3D7 intraerythrocytic forms by each compound were determined via a growth inhibition assay as described in Materials and Methods. falciparum parasites.Ī) IC 50s for inhibition of growth of P. Inhibition of growth and IC 50 determination of glucose uptake by isolated P. IC 50 concentrations and maximal inhibition for WU-1 and WU-2 for class I GLUT overexpressing cells. Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate. IC 50s were calculated using nonlinear regression analysis using GraphPad Prism 6.0 and are tabulated in Table 1. IC 50 determination of glucose uptake by HEK293 cells overexpressing human class I orthologues.Ģ-DG uptake in response to increasing WU-1 (A) or WU-2 (B) concentrations in HEK293 cells expressing the indicated GLUT protein. Uptake of 2-DG by HEK293 cells overexpressing hGLUT1-4 in the presence of the indicated compounds at indicated concentrations approximating their IC 50 for PfHT as shown in Fig 1. Specificity of hit compounds for PfHT over class I human orthologues. Chemical structures of the compounds tested are shown for comparison. Uptake data are expressed as means ± SEMs (n = 3).

IC 50s were calculated using nonlinear regression analysis IC 50 determinations using GraphPad Prism 6.0. Uptake of 2-DG by HEK293 cells overexpressing PfHT at increasing concentrations of the indicated compounds. IC 50 determination of glucose uptake by PfHT-overexpressing HEK293 cells.

Ic50 graphpad prism